Fluidigm®

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BioMark Instrument System
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EP-1 Instrument System
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Chips & Kits
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SlingShot
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Access Array
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Copy Number Variation
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Single Cell Gene Expression
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SNP Genotyping
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Gene Expression
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Fluidigm Application News

> Comprehensive Profiling of miRNAs in Stem Cells Using Microfluidics...

> Japanese Researchers Use Fluidigm's BioMark Platform for Stem Cell Expression Studies
– Genomeweb (6/30/09)

> Kyoto University's CiRA (Director, Dr. Yamanaka) Purchases Fluidigm BioMark™ System for Stem Cell Reprogramming Research
– Single Cell Study Holds the Key to iPS Progress (6/24/09)

> Keio University's Dr. Suda Selects Fluidigm Biomark™ System for Single Cell Research
– Single Cell Study Holds the Key to Hematopoietic Stem Cell Progress (6/22/09)

Related Products

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Lab901 | RNA Analysis
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SlingShot™ Kit from Fluidigm
Bringing the power of Digital PCR to next generation sequencing Sample Quantitation All current next-generation platforms rely on the ability to amplify individual library DNA molecules, it is this ability to amplify and then to read individual DNA molecules that allows massive throughput capabilities. The challenge to instrument operators is how to accurately and reproducibly determine library concentrations which will allow them to achieve single molecule amplification.
In the example shown i the diagram above the 454 workflow is used. If the concentration of the sample is overestimated the majority of the beads will be blanked resulting in a failed sequencing read. Conversely, if sample concentration is underestimated, the majority of the beads will contain multiple copies of DNA which will in turn cause a failed read. Sequencing throughput and data quality is maximized when the maximum number of emPCR beads contain exactly one DNA molecule.
Fluidigm Video Presentation | Click on the image (External Link)