SlingShot™ Kit from Fluidigm

Bringing the power of Digital PCR to next generation sequencing

The Digital Array Difference

• Obtain absolute measurement of your amplifiable sample
• Eliminate costly titration steps while improving data quality
• Use as little as 1 pg of material
• The Power of Digital PCR

SlingShot™ sample quantification is a novel application of digital PCR for absolute quantification of amplifiable DNA in your library. With SlingShot, you gain the power of digital PCR on digital arrays to find the optimal DNA-to-bead ratio for emPCR.

SlingShot Explained

SlingShot Kit uses digital PCR assays to detect only amplifiable molecules within the sample mixture. This absolute measure eliminates the need for mass-based calibrators or reference samples. In addition, because of digital PCR sensitivity, only minimal sample concentrations are required to achieve quantitative results. The accuracy and sensitivity offered by SlingShot allows you to sequence previously undetectable samples while saving time and money.

Each bright spot indicates a positive PCR reaction or an amplicon that can be sequenced. By counting the number of positive reactions the total copy number of amplicons in the samples of interest can be determined.