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Gene Expression
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The BioMark 48.48 Dynamic Array - Real Time PCR |
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Dynamic Array for Single Cell Gene Expression Analysis |
Many laboratories depend on TaqMan® real-time qPCR to validate biomarkers as clinically useful. These data "factories" have to maintain banks of robots and detection instruments to process tens of thousands of samples per year. The logistical friction associated with intensive material handling takes its toll on efficiency. More importantly, the quantities of reagents associated with microwell plates represent a significant cost burden. No wonder labs like these wish to cut back on instrumentation and say "goodbye" to microtitre plates.
The BioMark 48.48 Dynamic Array is a much more efficient solution for large-scale real-time qPCR. The key to this efficiency is the matrix of channels, chambers and integrated valves finely patterned into layers of silicone. This material is gas permeable, allowing the blind fill of fluids into valve-delimited chambers. The valves partition samples and reagents and allow them to be systematically combined into 2,304 reactions.
The significance of this approach to operational efficiency is immense. Managing a gene-expression study involving 2,000 samples against a set of 48 genes would require 1,000 96-well plates as compared to 42 dynamic arrays. Managing the same study would require 4,608 steps on microplates and only 96 liquid-transfer steps per chip. Comparative time required to complete such a study would typically involve 100 days on plates and just 4 1/2 days on chips. In addition to the above benefits, the running cost is reduced by half or more.
| Parameter | Specification |
| Sensitivity of Detection | Single-copy, per 10nL reaction |
| Input Volume per well | 5 microlitres (minimum) |
| Liquid transfer steps | only 96 |
| Reactions per chip | 2304 |
| Experiment tracking | Chip specific barcodes |
Dynamic Array
48.48
BioMark
Gene Expression
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