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DPO™ - Dual Priming Oligonucleotide Platform

• Single base discrimination
• No primer competition and dimersisation in multiplex assays
• Reproducible
• High specificity

Publication Reference >
 Dual Priming Oligonucleotide system for the
multiplex detection of respiratory viruses and
SNP genotyping of CYP2C19 gene
   
Slide Overview >
 DPO Chemistry

Dual Priming Oligonucleotide (DPO™) is structurally and fundamentally different from the conventional primers. It comprises of two separate priming regions (a first priming region and a second priming region) joined by a polydeoxyinosine linker. The linker forms like a "bubble-like structure" which itself is not involved in priming, but rather delineates the boundary between two parts of primer.

Assays available using DPO™

TapeStation:
Sepsis, Respiratory Viruses, Sexually Transmitted Infections, HPV, TB, Candida, HBV resistance typing, Pneumobacter, JAK2 Genotyping, FLT3 Genotyping, BCR/ABL, PNL/RaRa, AML1/ETO, MTHFR Genotyping, CYP2C19 Genotyping and ApoE Haplotyping.

Automated Capillary Sequencers
HPV Genotyping, Respiratory Pathogens, Sexually Transmitted Infections and HBV resistance typing.

TapeStation
Instrument
DNA Fragment Analyser

ABI 3130xl
instrument image
Fragment Analysis

Further Details

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