Seegene Research Reagents

DNA Walking SpeedUp™ Kit

DNA Walking SpeedUp™ kit provides information about
unknown sequences adjacent to upstream or downstream known sequences.

Previous methods commonly used for this purpose include inverse PCR (IPCR), ligation-mediated PCR (LM-PCR) and randomly primed PCR (RP-PCR).

Although these methods have been used efficiently and have proven to be effective, they are still hampered by laborious steps such as restriction enzyme digestion, adaptor ligation, and purification procedures. Moreover, arbitrary pimers in RP-PCR often result in non-specific products due to non-specific annealing.

These drawbacks of existing genomic walking methods can be overcome by employing Seegene's proprietary ACPTM (Annealing Control Primer)

Seegene developed DNA walking technology by incorporating ACP™ (Annealing Control Primer) and DPO™ (Dual Priming Oligonucleotide) technologies, which maximize the specificities of short primers and target specific primers, respectively, and furnishes optimized data about unknown sequences.

Note! DNA WalkingTM kit II, which provides widen application and higher success ratio than DNA WalkingTM kit I, has been recently released.

Upgrade!! DNA WalkingTM kit II.

Widen Application !!
Kit allows various vector mediated transgene integration site identification
as well as unknown sequence identification

Higher Success Ratio !!
Kit is highly optimized for unknown sequence identification

Longer PCR Product !!
Kit generates long PCR products that you can easily analyze

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