Fluidigm Access Array™ System

Introducing the Superior Sample Preparation System for Next-Generation Sequencing

	48 x 48 Access Array	IFC Contoller AX
		Thermal Cycler

Sample Capture and Target Enrichment

Sample capture and target enrichment refer to the ability to select a specific region of interest prior to sequencing. For example, if you were interested in examining 20 specific genes from a large cohort of individuals it would be both wasteful and prohibitively expensive to sample the entire genome of each individual. Instead, sample capture and target enrichment technologies allow you to select the specific areas of interest from each individual and thus only sequence that specific area of interest.

Sample Barcoding for Multiplexed Sequencing

One of the largest challenges facing next-generation sequencing operators today is how to utilize the massive amounts of throughput enabled by the new crop of instruments. While all of the systems in use today allow massive amounts of data to be generated on a per sample basis, they lack a simple and reliable method of running multiple samples per run, and thus harvesting the tremendous throughput of these instruments. Barcoding samples during the sample capture process enables the users to combine multiple samples per sequencing run, and then later during the data analysis step, determine which sample the resulting data came from.

Sequencing Library Preparation Using Amplicon Tagging

Library preparation for next-generation sequencing is by far the most time and labor demanding part of the entire next-generation sequencing process. While necessary for whole genome sequencing studies, the process can be almost entirely eliminated for deep resequencing projects through the use of amplicon tagging. By incorporating the adaptor sequences into the primer design the final PCR product is ready to go into emPCR or onto the flowcell since it already contains the necessary capture sequences.